Pernicious Anemia Cascade

Specimen:

Test Code

ACASM

Specimen Name

Pernicious Anemia Cascade

Collect

1-SST

Submit

3 mLs (Min 1.6 mLs) serum

Special Handling

Fasting: 12 hours, required, For 12 hours before specimen collection, patient should not take multivitamins or dietary supplements (eg, hair, skin, and nail supplements) containing biotin (vitamin B7), For 1 week before specimen collection, if medically feasible, patient should not take proton pump inhibitors (omeprazole, lansoprazole, dexlansoprazole, esomeprazole, pantoprazole, and rabeprazole), For at least 2 weeks before specimen collection, patient should not take or receive drugs that interfere with gastrointestinal motility (eg, opioids)

Rejection Criteria

Grossly hemolyzed, lipemic, icteric specimens

Stability

Frozen 30 days

Methodology

Competitive-Binding Immunoenzymatic Assay

Performed

Monday - Saturday

Reported

2-5 days

CPT Codes

  • 82607

Components

Includes: Vitamin B12 Assay, If reflexed any of the follwing could be performed Intrinsic Factor Blocking Ab, Gastrin test, Methylmalonic Acid

Additional Information

Patient Preparation: Ask patients if they have received a vitamin B12 injection or radiolabeled vitamin B12 injection within the last 2 weeks. Patient results will not reflect deficiency or malabsorption after recent B12 injection. If patient has received such an injection within the past 2 weeks, this test should not be ordered. https://www.mayocliniclabs.com/-/media/it-mmfiles/Special-Instructions/A/6/3/benign-hematology-test-request-form. Patient Preparation: 1. Fasting: 12 hours, required 2. For 12 hours before specimen collection, patient should not take multivitamins or dietary supplements (eg, hair, skin, and nail supplements) containing biotin (vitamin B7). 3. For 1 week before specimen collection, if medically feasible, patient should not take proton pump inhibitors (omeprazole, lansoprazole, dexlansoprazole, esomeprazole, pantoprazole, and rabeprazole). 4. For at least 2 weeks before specimen collection, patient should not take or receive drugs that interfere with gastrointestinal motility (eg, opioids).

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Common Lab Definitions

  • Serum 
  • Plasma 
  • STT