Acid Fast Bacillus Culture w/ AFB Stain

AFBCU

Acid Fast Bacillus Culture w/ AFB Stain

Sputum: early morning collection in a sterile leak-proof container.  Limit the number of samples to 3 specimens collected consecutively in 8- to 24-hour intervals, with at least one being an early morning specimen. Early morning sputum specimen is preferred, approval is required for additional specimens. The number of specimens that should be submitted is related to the results of early smear examinations. Sputum: early morning collection in a sterile leak-proof container. Limit the number of samples to 3 specimens collected consecutively in 8- to 24-hour intervals, with at least one being an early morning specimen. Early morning sputum specimen is preferred. Approval is required for additional specimens. The number of specimens that should be submitted is related to the results of early smear examinations.

Early morning urine in a sterile leak-proof container. Gastric aspirate, body fluid and tissue in a sterile leak-proof container.

5-20 mLs sputum, at  least 1-2 mLs CSF, 5-20 mLs gastric aspirate, >= 40mLs urine, 5-50 mLs body fluid, or 1 gram of tissue, All Refrigerated

Collection of early morning specimens of urine and sputum on each of 3 consecutive days is optimum. Gastric lavage specimens must be neutralized with sodium bicarbonate if transport delayed > 4 hours. Collect gastric aspirate 8 hours after food ingestion. Suggest collection of gastric aspirates for 3 consecutive days. Indicate source of specimen on the lab form. Tissue: submit skin lesions or other tissue; keep moistened with sterile saline. Indicate if Mycobacteria other than tuberculosis (MOTT) are suspected. This may expedite identification. Client is notified of positive stain/culture. Additional information to include when submitting a specimen for culture; patient's PPD status; BCG immunization history; prior anti-tuberculous therapy. Please note on test request form if sputum from a Cystic Fibrosis patient.

Frozen samples; Multiple same-site specimens (more than one in 24 hours; exception sputum (see collection instructions), dry material, non-sterile or leaking container, improper volume of sputum (<2 ml) or fluids (<1 ml), material collected and transported on a swab, and stool.

Refrigerated