ANA IFA with Reflex to Titer and ANA Autoantibody Panel

Specimen:

Test Code

ANAFLX

Specimen Name

ANA IFA with Reflex to Titer and ANA Autoantibody Panel

Collect

1-SST

Submit

3 mLs (Min: 1 mL) Serum Refrigerated

Special Handling

Separate serum from cells ASAP (within 8 hours)

Rejection Criteria

Body fluids. Heat inactivated, hemolyzed, lipemic or contaminated specimens.  

Stability

Ambient 8 hours; Refrigerated 7 days; Frozen >7 days

Methodology

Indirect fluorescence assay (IFA) using HEp-2 cells; ANA Panel: Multiplex Flow Immunoassay

Performed

Monday - Friday by 0700

Reported

2-4 days

CPT Codes

  • 86255
  • If positive titer add 86256
  • if positive add 86235 x10
  • ds DNA 86225

Components

Includes: antibody screen, a quantitative Anti-dsDNA result and qaulitative results for Anti-Chromatin, Anti-Ribosomal P, Anti-SS-A (52kD and 60kD), Anti-SS-B, Anti-Sm/RNP, Anti-RNP (A and 68), Anti-Scl-70, Anti-Jo-1, and Anti-Centromere B.

Additional Information

The ANA panel includes an antibody screen, a quantitative Anti-dsDNA result and qualitative results for Anti-Chromatin, Anti-Ribosomal P, Anti-SS-A (52kD and 60kD), Anti-SS-B, Anti-Sm/RNP, Anti-RNP (A and 68), Anti-Scl-70, Anti-Jo-1, and Anti-Centromere B.

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Test Information

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Common Lab Definitions

  • Serum 
  • Plasma 
  • STT