- ANAH2G
ANA, IgG by ELISA w/reflex to ANA, HEp2 Substrate
Specimen:
Test Code
ANAH2G
Specimen Name
ANA, IgG by ELISA w/reflex to ANA, HEp2 Substrate
Collect
1-SST
Submit
1 mL (Min: 0.5 mL) Serum Refrigerated
Special Handling
Separate serum from cells ASAP or within 2 hours of collection.
Rejection Criteria
Non-serum, heat inactivated, grossly hemolyzed, or severely lipemic specimens.
Stability
Ambient 48 hours; Refrigerated 14 days; Frozen 1 year (avoid repeated freeze/thaw cycles)
Methodology
Qualitative Enzyme-Linked Immunosorbent Assay/Semi-Quantitative Indirect Fluorescent Antibody
Performed
Sunday - Saturday
Reported
2-5 days
CPT Codes
- 86038
- if reflexed
- add 86039
Components
If ANA are detected by ELISA, then Antinuclear Antibody (ANA), HEp-2, IgG by IFA will be added.
Additional Information
ANA identified by indirect fluorescence assay (IFA) using HEp-2 substrate and IgG-specific conjugate at a screening dilution of 1:80. Positive nuclear patterns reported include homogeneous, speckled, centromere, nucleolar, or nuclear dots. Positive cytoplasmic patterns reported include reticular/AMA, discrete/GW body-like, polar/golgi-like, rods and rings, or cytoplasmic speckled patterns. All positive results are reported with endpoint titers at no additional charge.
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Test Information
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Common Lab Definitions
- Serum
- Plasma
- STT