- ANGER
ANA, IgG by ELISA w/reflex to ANA, HEp2 & ENA
Specimen:
Test Code
ANGER
Specimen Name
ANA, IgG by ELISA w/reflex to ANA, HEp2 & ENA
Collect
1-SST
Submit
2 mLs (Min: 1 mL) Serum Refrigerated
Special Handling
Separate serum from cells ASAP or within 2 hours of collection.
Rejection Criteria
Nonserum specimens. Contaminated, grossly hemolyzed, heat-inactivated, severely lipemic, specimens, or inclusion of fibrin clots.
Stability
Ambient 48 hours; Refrigerated 14 days; Frozen 1 month (avoid repeated freeze/thaw cycles)
Methodology
Qualitative Enzyme-Linked Immunosorbent Assay (ELISA) / Semi-Quantitative Indirect Fluorescent Antibody (IFA) / Semi-Quantitative Multiplex Bead Assay / Semi-Quantitative Enzyme-Linked Immunosorbent Assay (ELISA)
Performed
Sunday - Saturday
Reported
2-7 days
CPT Codes
- 86038
- if reflexed
- add 86039
- if reflexed
- add 86235 x7 and 86225
- if reflexed
- add 86256
Components
Specimens are screened for ANA using ELISA. If ANA IgG is detected by ELISA, then Antinuclear Antibody (ANA), HEp-2, IgG by IFA will be added. If ANA, IgG by IFA is confirmed positive with a titer of 1:80 or greater, then a titer and pattern will be reported. In addition, samples positive for ANA, IgG by IFA will reflex to Double-Stranded DNA (dsDNA) Antibody, IgG by ELISA; Jo-1 Antibody, IgG; Smith/RNP (ENA) Antibody, IgG; Scleroderma (Scl-70) (ENA) Antibody, IgG; Smith (ENA) Antibody, IgG; SSA 52 and 60 (Ro) (ENA) Antibodies, IgG; and SSB (La) (ENA) Antibody, IgG. If Double-Stranded DNA (dsDNA) Antibody, IgG by ELISA is detected, then Double-Stranded DNA (dsDNA) Antibody, IgG by IFA (using Crithidia luciliae) will be added. ANA identified by indirect fluorescence assay (IFA) using HEp-2 substrate and IgG-specific conjugate at a screening dilution of 1:80. Positive nuclear patterns reported include homogeneous, speckled, centromere, nucleolar, or nuclear dots. Positive cytoplasmic patterns reported include reticular/AMA, discrete/GW body-like, polar/golgi-like, rods and rings, or cytoplasmic speckled patterns. All positive results are reported with endpoint titers at no additional charge.
Additional Information
—
Disclaimer:
It is preferable to locate tests on the website and not rely on printed test information, which may soon be out of date. Updated test information can only be guaranteed by using this website.
Choose the Right Test
Test Information
How to Label Your Sample
- Label Your Test 1
- Label Your Test 2
- Label Your Test 3
- Label Your Test 4
- Label Your Test 5
Common Test Phrases
- Label Your Test 1
- Label Your Test 2
- Label Your Test 3
- Label Your Test 4
- Label Your Test 5
Common Lab Definitions
- Serum
- Plasma
- STT