BRAF Mutation

Specimen:

Test Code

YBRAF

Specimen Name

BRAF Mutation

Collect

Parrafin embedded formalin fixed tissue; Bone Marrow Aspirate

Submit

Paraffin embedded formalin fixed tissue block, or 5 Unstained slides- 10µm thick with ≥20% tumor (with one adjacent H&E).  Minimum volume: 5mmX5mm area if tissue is 100% tumor; 10mmX10mm if tissue is 50% tumor

Special Handling

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Rejection Criteria

Paraffin blocks with ≤10% tumor. Tissue fixed in heavy metal fixative or decalcified tissue.
Heparin, NaHep (green-top) tubes.

Stability

Transport blocks at room temp, in summer months when hot, transport block on cold packs. Refrigerate peripheral blood and bone marrow aspirate.

Methodology

Pyrosequencing

Performed

Monday - Friday

Reported

11-15 days

CPT Codes

  • 81210

Components

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Additional Information

The test is currently validated and recommended for mutation detection in colorectal adenocarcinomas, melanomas and papillary thyroid cancer.

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Common Lab Definitions

  • Serum 
  • Plasma 
  • STT