- YLEADBS
Lead, Filter Paper Blood Spot
Specimen:
Test Code
YLEADBS
Specimen Name
Lead, Filter Paper Blood Spot
Collect
Whole blood spotted on Whatman 903 sample collection paper
Submit
Fill and saturate 3 complete spots. Send ambient
Special Handling
Circles on filter paper must be completely saturated through.
Collection Procedure:
Collection area and materials must be lead free and protected from contamination.
Collector must thoroughly wash hands and wear powder-free gloves.
Ensure patient's hands are washed and warm. Fingers and hands that are visibly dirty should first be washed with soapy water, rinsed with clean water, and dried with a clean low lint towel preferably plain white with no printed design if using paper towels. Cleanse puncture site with an alcohol wipe and allow to air dry. The puncture site can be prepped using only soap and water as stated above if the use of alcohol is contraindicated.
Perform capillary puncture. Use a puncture device that delivers a lacerating puncture, which cuts across the capillary bed. Avoid devices designed for glucose or hemoglobin monitoring where only one drop of blood is needed.
Wipe away first drop of blood. Apply gentle pressure to allow a second large drop of blood to form. Lightly touch the printed side of filter paper card to the large blood drop and fill each printed circle with a SINGLE application of blood. Allow the blood to soak through completely filling the circle. Observe the saturation of each printed circle as the blood flows through the filter paper. Do not layer blood spot. Do not touch filter paper to skin. Do not apply blood to both sides of filter paper.
Label specimen completely with patients name, DOB, collection date and time.
Close the collection device and allow blood specimen to AIR DRY THOROUGHLY on a flat surface. Do not heat or allow the blood spots on the filter paper to touch other surfaces during the drying process.
Place dried filter paper along with a complete blood lead requisition in the biohazard transport bag.
Blood Lead Collection Cautions:
Failure to allow puncture site to air dry may cause the specimen to be diluted.
Milking or squeezing the puncture may cause hemolysis and add mixture of tissue fluids with specimen.
Do not layer successive drops of blood on the circle spot. If blood flow diminishes to incompletely fill circles repeat the capillary puncture and repeat collection using a new filter paper card.
Avoid touching area within circle before collection and blood spots after collection on filter paper. Do not allow water, feeding formulas, antiseptic solutions, etc. to come into contact with the sample.
Do not place filter paper in the envelope until thoroughly dry.
Insufficient drying can adversely affects test results.
Collection Procedure:
Collection area and materials must be lead free and protected from contamination.
Collector must thoroughly wash hands and wear powder-free gloves.
Ensure patient's hands are washed and warm. Fingers and hands that are visibly dirty should first be washed with soapy water, rinsed with clean water, and dried with a clean low lint towel preferably plain white with no printed design if using paper towels. Cleanse puncture site with an alcohol wipe and allow to air dry. The puncture site can be prepped using only soap and water as stated above if the use of alcohol is contraindicated.
Perform capillary puncture. Use a puncture device that delivers a lacerating puncture, which cuts across the capillary bed. Avoid devices designed for glucose or hemoglobin monitoring where only one drop of blood is needed.
Wipe away first drop of blood. Apply gentle pressure to allow a second large drop of blood to form. Lightly touch the printed side of filter paper card to the large blood drop and fill each printed circle with a SINGLE application of blood. Allow the blood to soak through completely filling the circle. Observe the saturation of each printed circle as the blood flows through the filter paper. Do not layer blood spot. Do not touch filter paper to skin. Do not apply blood to both sides of filter paper.
Label specimen completely with patients name, DOB, collection date and time.
Close the collection device and allow blood specimen to AIR DRY THOROUGHLY on a flat surface. Do not heat or allow the blood spots on the filter paper to touch other surfaces during the drying process.
Place dried filter paper along with a complete blood lead requisition in the biohazard transport bag.
Blood Lead Collection Cautions:
Failure to allow puncture site to air dry may cause the specimen to be diluted.
Milking or squeezing the puncture may cause hemolysis and add mixture of tissue fluids with specimen.
Do not layer successive drops of blood on the circle spot. If blood flow diminishes to incompletely fill circles repeat the capillary puncture and repeat collection using a new filter paper card.
Avoid touching area within circle before collection and blood spots after collection on filter paper. Do not allow water, feeding formulas, antiseptic solutions, etc. to come into contact with the sample.
Do not place filter paper in the envelope until thoroughly dry.
Insufficient drying can adversely affects test results.
Rejection Criteria
Rough or wrinkled spot or paper.
Quantity of sample not sufficient for testing.
Apparent diluted blood spot.
Multiple spotting.
Quantity of sample not sufficient for testing.
Apparent diluted blood spot.
Multiple spotting.
Stability
Ambient 6 months
Methodology
ICP-MS
Performed
Monday /Wednesday/Friday
Reported
1-4 days
CPT Codes
- 83655
Components
—
Additional Information
Recommended Use:
Recommended for routine testing for lead exposure in pediatric populations.
Confirm elevated results with a venous sample.
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