Thrombin Time

YTT

Thrombin Time

Sodium Citrate Whole blood OR Sodium Citrate Plasma

1 Sodium citrate whole blood tube, Ambeint (Must be received at the lab within 4 hours).
OR
2 - 0.5 mL aliquots (Min: 1- 0.5 mL aliqout), Frozen.

Tubes MUST be full. DO NOT overfill or underfill . MUST be mixed immediately after filling with blood. Gently mix tube by inverting 5 to 6 times. Vigorous mixing or shaking may cause hemolysis, which will affect the result. Using a plastic disposable pipette, carefully transfer the top ¾ of the plasma into a polypropylene tube, avoiding the buffy coat. DO NOT pour off. DO NOT PUT ALL PLASMA IN ONE ALIQUOT TUBE
Centrifuge and test within 4 hours. If testing cannot be completed within 4 hours, prepare platelet poor plasma and freeze

Specimens collected in an anticoagulant other than 3.2% sodium citrate
Under or overfilled specimens
Clotted specimens
Grossly hemolyzed, lipemic, or icteric specimens
Refrigerated or frozen whole blood specimens

Room temperature 4 hours ; Frozen:1 month If specimen cannot be tested within 4 hours, prepare platelet poor citrated plasma and freeze plasma in 0.5 mL aliquots